Welcome to Fly Facility !
Using our services you can rapidly generate both P-element and PhiC31 integrase-mediated site-specific transgenesis.
Drosophila lines carrying different reporter gene constructs, expression constructs and test gene function by dsRNA microinjection. Fly-Facility associated with a research lab has a longstanding experience in Drosophila transgenesis. Our device : Quality, Rapidity, Flexibility and Competitive pricing.

Welcome !
Intersectional lines
Generation and phenotyping of a new collection of Drosophila transgenic lines dedicated to intersectional approaches

We are planning to generate and phenotype a collection of transgenic lines carrying zip-LexA-Activation Domain which then can be combined with GAL4 line collections (such as Janelia Farm collection) to refine transgene expression by intersectional approaches (Ting et al., Genetics 188, 229-233, 2011). We are planning to use InSITE swapable vectors to generate our collection so that the zip-LexA-Activation Domain cassette can be easily exchanged to all other casettes from InSITE collection (Gohl et al., 2011, Nat Methods 8, 231-237).
We will then phenotype the generated zip-LexA-AD collection in embryos and in the adult fly brain (collaboration with F. Rouyer lab). The generated image data will be annotated and available via dedicated and searchable database.

Image Exemple

  • Coupling with GAL4

 Image Exemple




  • InSITE system: Integrase swappable in vivo targeting element
    (in vivo swapping approach for enhancer trapping)

Image Exemple

 
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