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Using our services you can rapidly generate both P-element and site-specific transgenic Drosophila lines carrying different reporter gene constructs, expression constructs and test gene function by dsRNA microinjection. More than 1000 transgenic lines generated and more than 200 dsRNAs injected and analysed. Our device : Quality, Rapidity and Competitive pricing.
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Generation and phenotyping of a new collection of Drosophila transgenic lines dedicated to intersectional approaches

We are planning to generate and phenotype a collection of transgenic lines carrying zip-LexA-Activation Domain which then can be combined with GAL4 line collections (such as Janelia Farm collection) to refine transgene expression by intersectional approaches (Ting et al., Genetics 188, 229-233, 2011). We are planning to use InSITE swapable vectors to generate our collection so that the zip-LexA-Activation Domain cassette can be easily exchanged to all other casettes from InSITE collection (Gohl et al., 2011, Nat Methods 8, 231-237).
We will then phenotype the generated zip-LexA-AD collection in embryos and in the adult fly brain (collaboration with F. Rouyer lab). The generated image data will be annotated and available via dedicated and searchable database.

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  • Coupling with GAL4

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  • InSITE system: Integrase swappable in vivo targeting element
    (in vivo swapping approach for enhancer trapping)

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